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Allen Institute for Brain Science cre-inducible gfp aav
PMV LepR projection sites express GUCY2C protein in projections. (a) Unilateral stereotaxic injection of cre-inducible <t>GFP</t> <t>AAV</t> into the PMV of LepR-cre mice reveals GFP(+) projections in hypothalamic and extrahypothalamic nuclei (Allen Institute for Brain Science, experiment ID 167656388)(Oh et al. 2014). (b) GUCY2C is expressed in PMV projection sites of LepR neurons, but not with TH. (c) High magnification reveals that GUCY2C protein is expressed in projections, not neuronal cell bodies in hypothalamic nuclei other than the PMV Scale bars in (b): 200 μm; in C: 20 μm. LSV: lateral septal nucleus, ventral part; BST: bed nucleus of the stria terminalis; MPO: medial preoptic nucleus; vl: ventrolateral aspect of the ventromedial hypothalamus; ARC: Arcuate nucleus; SNc: substantia nigra, pars compacta; PA: posterior amygdalar nucleus. Source images for panel (a) can be found at the following links:
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10X Genomics d f g rosa loxpstoploxp sun1gfp sir n2c cre aav g nucflag facs gfp
PMV LepR projection sites express GUCY2C protein in projections. (a) Unilateral stereotaxic injection of cre-inducible <t>GFP</t> <t>AAV</t> into the PMV of LepR-cre mice reveals GFP(+) projections in hypothalamic and extrahypothalamic nuclei (Allen Institute for Brain Science, experiment ID 167656388)(Oh et al. 2014). (b) GUCY2C is expressed in PMV projection sites of LepR neurons, but not with TH. (c) High magnification reveals that GUCY2C protein is expressed in projections, not neuronal cell bodies in hypothalamic nuclei other than the PMV Scale bars in (b): 200 μm; in C: 20 μm. LSV: lateral septal nucleus, ventral part; BST: bed nucleus of the stria terminalis; MPO: medial preoptic nucleus; vl: ventrolateral aspect of the ventromedial hypothalamus; ARC: Arcuate nucleus; SNc: substantia nigra, pars compacta; PA: posterior amygdalar nucleus. Source images for panel (a) can be found at the following links:
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Image Search Results


PMV LepR projection sites express GUCY2C protein in projections. (a) Unilateral stereotaxic injection of cre-inducible GFP AAV into the PMV of LepR-cre mice reveals GFP(+) projections in hypothalamic and extrahypothalamic nuclei (Allen Institute for Brain Science, experiment ID 167656388)(Oh et al. 2014). (b) GUCY2C is expressed in PMV projection sites of LepR neurons, but not with TH. (c) High magnification reveals that GUCY2C protein is expressed in projections, not neuronal cell bodies in hypothalamic nuclei other than the PMV Scale bars in (b): 200 μm; in C: 20 μm. LSV: lateral septal nucleus, ventral part; BST: bed nucleus of the stria terminalis; MPO: medial preoptic nucleus; vl: ventrolateral aspect of the ventromedial hypothalamus; ARC: Arcuate nucleus; SNc: substantia nigra, pars compacta; PA: posterior amygdalar nucleus. Source images for panel (a) can be found at the following links:

Journal: Brain structure & function

Article Title: Two Distinct GUCY2C Circuits with PMV (Hypothalamic) and SN/VTA (Midbrain) Origin

doi: 10.1007/s00429-019-01949-y

Figure Lengend Snippet: PMV LepR projection sites express GUCY2C protein in projections. (a) Unilateral stereotaxic injection of cre-inducible GFP AAV into the PMV of LepR-cre mice reveals GFP(+) projections in hypothalamic and extrahypothalamic nuclei (Allen Institute for Brain Science, experiment ID 167656388)(Oh et al. 2014). (b) GUCY2C is expressed in PMV projection sites of LepR neurons, but not with TH. (c) High magnification reveals that GUCY2C protein is expressed in projections, not neuronal cell bodies in hypothalamic nuclei other than the PMV Scale bars in (b): 200 μm; in C: 20 μm. LSV: lateral septal nucleus, ventral part; BST: bed nucleus of the stria terminalis; MPO: medial preoptic nucleus; vl: ventrolateral aspect of the ventromedial hypothalamus; ARC: Arcuate nucleus; SNc: substantia nigra, pars compacta; PA: posterior amygdalar nucleus. Source images for panel (a) can be found at the following links:

Article Snippet: In both circuits, GUCY2C appears to be transcribed (mRNA) and translated (protein) in neuronal cell bodies, and then distributed along the length of axons, which project to other nuclei (no mRNA; only protein). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3. caption a7 PMV LepR projection sites express GUCY2C protein in projections. (a) Unilateral stereotaxic injection of cre-inducible GFP AAV into the PMV of LepR-cre mice reveals GFP(+) projections in hypothalamic and extrahypothalamic nuclei (Allen Institute for Brain Science, experiment ID 167656388)( Oh et al. 2014 ). (b) GUCY2C is expressed in PMV projection sites of LepR neurons, but not with TH. (c) High magnification reveals that GUCY2C protein is expressed in projections, not neuronal cell bodies in hypothalamic nuclei other than the PMV Scale bars in (b): 200 μm; in C: 20 μm.

Techniques: Injection